[HTML][HTML] 3D-printed, pocket-size diffusion cells for skin permeation investigation
Proceedings, 2018•mdpi.com
Here we present a novel, compact 3D-printed diffusion cell as an in vitro tool for skin
permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of
a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and
characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated
via MTT assays. The real-time diffusion of the molecule by means of fluorescence
microscopy allowed the determination of its diffusivity through the skin (~ 2· 10− 10 m2/s) …
permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of
a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and
characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated
via MTT assays. The real-time diffusion of the molecule by means of fluorescence
microscopy allowed the determination of its diffusivity through the skin (~ 2· 10− 10 m2/s) …
Here we present a novel, compact 3D-printed diffusion cell as an in vitro tool for skin permeation investigation. As proof-of-concept, a diffusion cell for studying the permeation of a model molecule (FITC-dextran, 4 kDa) through explanted mice skin is fabricated and characterized. Good viability of the tissue up to 24 h incubation in the cell is demonstrated via MTT assays. The real-time diffusion of the molecule by means of fluorescence microscopy allowed the determination of its diffusivity through the skin (~2·10−10 m2/s). Our results open the door for the real-time, high-throughput and cost-effective investigation of skin in any labs.
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