Primary culture and plasmid electroporation of the murine organ of Corti.

M Parker, A Brugeaud, ASB Edge - JoVE (Journal of Visualized …, 2010 - jove.com
In all mammals, the sensory epithelium for audition is located along the spiraling organ of
Corti that resides within the conch shaped cochlea of the inner ear (fig 1). Hair cells in the
developing cochlea, which are the mechanosensory cells of the auditory system, are aligned
in one row of inner hair cells and three (in the base and mid-turns) to four (in the apical turn)
rows of outer hair cells that span the length of the organ of Corti. Hair cells transduce sound-
induced mechanical vibrations of the basilar membrane into neural impulses that the brain …