In vivo selection of T-cell receptor junctional region sequences by HLA-A2 human T-cell lymphotropic virus type 1 Tax11-19 peptide complexes
M Saito, GP Taylor, A Saito, Y Furukawa… - Journal of …, 2001 - Am Soc Microbiol
M Saito, GP Taylor, A Saito, Y Furukawa, K Usuku, JN Weber, M Osame, CRM Bangham
Journal of Virology, 2001•Am Soc MicrobiolUsing HLA-peptide tetrameric complexes, we isolated human T-cell lymphotrophic virus type
1 Tax peptide-specific CD8+ T cells ex vivo. Antigen-specific amino acid motifs were
identified in the T-cell receptor Vβ CDR3 region of clonally expanded CD8+ T cells. This
result directly confirms the importance of the CDR3 region in determining the antigen
specificity in vivo.
1 Tax peptide-specific CD8+ T cells ex vivo. Antigen-specific amino acid motifs were
identified in the T-cell receptor Vβ CDR3 region of clonally expanded CD8+ T cells. This
result directly confirms the importance of the CDR3 region in determining the antigen
specificity in vivo.
Abstract
Using HLA-peptide tetrameric complexes, we isolated human T-cell lymphotrophic virus type 1 Tax peptide-specific CD8+ T cells ex vivo. Antigen-specific amino acid motifs were identified in the T-cell receptor Vβ CDR3 region of clonally expanded CD8+ T cells. This result directly confirms the importance of the CDR3 region in determining the antigen specificity in vivo.
American Society for Microbiology