Single-cell transcriptomics combined with interstitial fluid proteomics defines cell type–specific immune regulation in atopic dermatitis
TB Rojahn, V Vorstandlechner, T Krausgruber… - Journal of Allergy and …, 2020 - Elsevier
Background Atopic dermatitis (AD) is the most common chronic inflammatory skin disease,
but its complex pathogenesis is only insufficiently understood, resulting in still limited
treatment options. Objective We sought to characterize AD on both transcriptomic and
proteomic levels in humans. Methods We used skin suction blistering, a painless and
nonscarring procedure that can simultaneously sample skin cells and interstitial fluid. We
then compared results with conventional biopsies. Results Suction blistering captured …
but its complex pathogenesis is only insufficiently understood, resulting in still limited
treatment options. Objective We sought to characterize AD on both transcriptomic and
proteomic levels in humans. Methods We used skin suction blistering, a painless and
nonscarring procedure that can simultaneously sample skin cells and interstitial fluid. We
then compared results with conventional biopsies. Results Suction blistering captured …
