[HTML][HTML] Quantification of ADP and ATP receptor expression in human platelets
L Wang, O Östberg, AK Wihlborg, H Brogren… - Journal of Thrombosis …, 2003 - Elsevier
The mechanism of ADP-mediated platelet activation has been difficult to unravel due to the
large number of receptors for extracellular nucleotides (P2 receptors). mRNA levels in
circulating platelets are very low, but have been shown to be translationally active. By
optimizing mRNA extraction and using real time (RT)-PCR we were able to establish a
protocol for highly sensitive platelet mRNA quantification in human regular blood samples.
In platelets from healthy volunteers, only P2X 1, P2Y 1 and P2Y 12 were found in significant …
large number of receptors for extracellular nucleotides (P2 receptors). mRNA levels in
circulating platelets are very low, but have been shown to be translationally active. By
optimizing mRNA extraction and using real time (RT)-PCR we were able to establish a
protocol for highly sensitive platelet mRNA quantification in human regular blood samples.
In platelets from healthy volunteers, only P2X 1, P2Y 1 and P2Y 12 were found in significant …
