Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
The direct detection of antigen-specific T cells using tetramers of soluble peptide–major
histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical
immunology. However, the number of specificities that can be assessed simultaneously has
been a major limitation. Here we describe and validate a method using combinations of
fluorescent pMHC tetramers to simultaneously detect and enrich for many (≥ 15) T-cell
specificities in a single human blood sample.
histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical
immunology. However, the number of specificities that can be assessed simultaneously has
been a major limitation. Here we describe and validate a method using combinations of
fluorescent pMHC tetramers to simultaneously detect and enrich for many (≥ 15) T-cell
specificities in a single human blood sample.
Abstract
The direct detection of antigen-specific T cells using tetramers of soluble peptide–major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (≥15) T-cell specificities in a single human blood sample.
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