[HTML][HTML] Structural reevaluation of Streptococcus pneumoniae lipoteichoic acid and new insights into its immunostimulatory potency
N Gisch, T Kohler, AJ Ulmer, J Müthing, T Pribyl… - Journal of biological …, 2013 - ASBMB
Streptococcus pneumoniae is a Gram-positive human pathogen with a complex lipoteichoic
acid (pnLTA) structure. Because the current structural model for pnLTA shows substantial
inconsistencies, we reinvestigated purified and, more importantly, O-deacylated pnLTA,
which is most suitable for NMR spectroscopy and electrospray ionization-MS spectrometry.
We analyzed pnLTA of nonencapsulated pneumococcal strains D39Δcps and TIGR4Δcps,
respectively. The data obtained allowed us to (re) define (i) the position and linkage of the …
acid (pnLTA) structure. Because the current structural model for pnLTA shows substantial
inconsistencies, we reinvestigated purified and, more importantly, O-deacylated pnLTA,
which is most suitable for NMR spectroscopy and electrospray ionization-MS spectrometry.
We analyzed pnLTA of nonencapsulated pneumococcal strains D39Δcps and TIGR4Δcps,
respectively. The data obtained allowed us to (re) define (i) the position and linkage of the …